#ICAclust: a tool for clustering of temporal RNA-Seq data

rm(list = ls())
#Required package
install.packages("fastICA")
library(fastICA)

#Specifying data Path 
setwd("Your Path")

#Data (The first and second colum are genes Ids)
data <- read.table("https://det.ufv.br/~moyses/arq/RNA_SEQ_PIG.txt",
                   header = T, sep = " ")

#Function to clustering genes

ICAclust<-function(data=data, cluster_method="ward.D", mojena=3.75, times = c(20,40,70,90))
{            
X=data
Y=X[,3:ncol(X)]
X.ICA<-fastICA(Y,n.comp=4, alg.typ = "parallel", fun = "exp", alpha = 1.0,
               method = "C", row.norm = FALSE, maxit = 2000, tol = 1e-03, verbose = TRUE)
hc.ICA<-hclust(dist(X.ICA$S), method = cluster_method, members=NULL)
dendro<-as.dendrogram(hc.ICA)
plot(hc.ICA,labels = NULL, hang = 0.0,
     axes = TRUE, frame.plot = FALSE, ann = TRUE,
     main = "Cluster Dendrogram",
     sub = NULL, xlab = "", ylab = "Height")
hc.ICA$order
mojema=mean(hc.ICA$height)+mojena*sd(hc.ICA$height)
g=length(hc.ICA$height[hc.ICA$height>mojema]) + 1
rect.hclust(hc.ICA, k =g)
group_number<-g
group<-cutree(hc.ICA, k=g)
number_gene<-table(group)
grupos1<-as.data.frame(X$geneID)
comp<-cbind(group,grupos1)
dadosnovos <-cbind(group,X[,-c(1,2)])
dadosnovoss <-cbind(group,X[,2],X[,-c(1,2)])

#depends of temporal observation number
names(dadosnovoss)<-c("group","geneID","log2.21","log2.40","log2.70","log2.90") 
dadosnovos1 <-as.data.frame(dadosnovos)
a1=by(dadosnovos1, dadosnovos1[,"group"],function(x) colMeans(x))
x1<-do.call("rbind",as.list(a1))
write.csv(dadosnovoss, file = "groups.csv")
write.table(dadosnovoss, file = "groups.txt")
write.csv(x1, file = "averagevalues.csv")
write.table(x1, file = "averagevalues.txt")
result<-list( "nGroups" = group_number, "gbGroup"= number_gene)
return(result)
}

#Using ICAclust function
result <- ICAclust(data=data, cluster_method="ward.D", mojena=3.75, times = c(20,40,70,90))
result

##Graphic Function

graphicICAclust<-function(nrow,ncol,data1,data2,nGroups,times)
{
  data2<- data2[,-2]
  par(family="serif",mfrow=c(nrow,ncol))
  times<-times
  lim<-max(abs(c(round(min(data1[,-1])),round(max(data1[,-1])))))
  for (i in 1:nGroups)
  {
    plot(times,data1[i,-1],xlab="Times",
         ylab="fold-change",type="l",ylim=c(-lim,lim),main=" Average Pattern ")
    abline(h=0,lty=3)
  }
    lim<-max(abs(c(round(min(data2[,-1])),round(max(data2[,-1])))))
  for (i in 1:nGroups)
  {
    g1<-data2[data2$group==i,]
    matplot(t(g1[,2:ncol(g1)]), type = "l",col="black", xlab="Times",
            ylab="fold-change")
    abline(h=0,lty=3)
  }
}
#Reading files saved by ICAclust function
data1 <- read.table("averagevalues.txt", h=T)
data2 <- read.table("groups.txt", h=T)

#Using graphic function
graphicICAclust(2,3,data1,data2, result$nGroups, times=c(20,40,70,90))

rowID	     geneID	          log2.21	  log2.40         log2.70	   log2.90
1	ENSSSCT00000000131	-0.314227836	1.108112572	-1.413302448	-0.022367813
2	ENSSSCT00000000179	0.885541275	-1.596935142	-1.434095835	-2.584962501
3	ENSSSCT00000000201	0.569728337	0.419703753	-0.256698708	-0.039698327
4	ENSSSCT00000000203	0.416681844	0.423865082	-0.252498245	-0.144532967
5	ENSSSCT00000000207	0.512669343	0.426754424	-0.143655751	-0.072041678
6	ENSSSCT00000000220	0.341658605	0.687010654	-0.970983926	-0.825618251
7	ENSSSCT00000000280	1.485426827	0.722734208	-2.078002512	-1.394278939
8	ENSSSCT00000000296	0.944858446	1.332983283	-3.584962501	-2.321928095
9	ENSSSCT00000000383	0.682285451	0.946854335	-1.146031795	-0.847996907
10	ENSSSCT00000000438	0.278596901	0.452259012	-1.031271462	-0.96210016
11	ENSSSCT00000000457	-0.921144579	-0.257725494	-0.120854395	0.337264405
12	ENSSSCT00000000494	-1.062860024	-0.835937238	0.052046748	0.133624564
13	ENSSSCT00000000548	-0.437267447	-0.388161793	0.62058641	-1.409644241
14	ENSSSCT00000000578	0.626885671	0.786496317	0.599246727	0.284494768
15	ENSSSCT00000000665	-0.623436649	-0.719892081	1	        1.5360529
16	ENSSSCT00000000668	0.568962022	1.171227661	-1.217591435	-1.500229452
17	ENSSSCT00000000756	0.563397151	0.885027839	-1.183716533	0.175972178
18	ENSSSCT00000000798	1.262056639	0.881355504	0.885471612	0.043943348
19	ENSSSCT00000000835	-0.811238357	-0.20069735	0.421826665	0.584962501
20	ENSSSCT00000000912	-0.10346332	0.497136519	0.518467089	0.142527766
21	ENSSSCT00000000913	-0.096330079	0.491319022	0.516662522	0.142527766
22	ENSSSCT00000000967	1.150103561	-0.514573173	3.672425342	4.485426827
23	ENSSSCT00000001000	-0.971321247	-0.089039293	-1.053909164	-0.117771459
24	ENSSSCT00000001001	-3.807354922	1.460051111	-2.43171624	0.113656782
25	ENSSSCT00000001118	-0.361200249	-2.130847479	3	       -2.146841388
26	ENSSSCT00000001151	-0.291399297	-0.345774837	0.370837695	-1.614108846
27	ENSSSCT00000001275	-0.075725873	0.837873017	-1.503412573	0.55307844
28	ENSSSCT00000001460	-0.512061954	1.209923069	-1.777607579	1.523561956
29	ENSSSCT00000001480	-0.449610648	1.013175389	1.011227255	0.553935605
30	ENSSSCT00000001555	1.594920746	1.240654628	1.012900489	0.244887059
31	ENSSSCT00000001675	0.65041213	1.214225609	-1.112321025	-0.035697369
32	ENSSSCT00000001836	0.736965594	1.058893689	1.180896119	0.800130595
33	ENSSSCT00000001909	-0.37367847	-0.227951947	0.345135486	-1.250961574
34	ENSSSCT00000002021	1.874469118	-1.479992941	1	        1.807354922
35	ENSSSCT00000002052	2.517058436	-1.779480124	-4.564784619	-2.187627003
36	ENSSSCT00000002146	1.304854582	1.862496476	0.425305835	2.039528364
37	ENSSSCT00000002196	0.964810572	-1.058495649	-2.209453366	-1.207804885
38	ENSSSCT00000002203	-0.557426551	-0.591960227	-0.058214614	-1.014241923
39	ENSSSCT00000002288	-0.360023397	-0.026123587	-1.280980746	-0.790118217
40	ENSSSCT00000002511	-0.352248546	-0.781165398	0.08061846	-1.184424571
41	ENSSSCT00000002535	-1.530514717	1.054447784	0.391167548	-0.845614255
42	ENSSSCT00000002551	-0.474371639	-0.376209756	-1.509674373	-1.335603032
43	ENSSSCT00000002652	2.087462841	0.791682764	-1.389566812	0.943416472
44	ENSSSCT00000002818	-0.433483167	-0.351101855	0.454258809	0.28567221
45	ENSSSCT00000002833	0.825042921	0.758806643	-1.417017863	0.222392421
46	ENSSSCT00000002989	0.758049732	1.076535506	1.425860469	1.098302074
47	ENSSSCT00000002998	-1	        -0.27676638	-0.154534693	-0.839903616
48	ENSSSCT00000003039	-0.282862615	-0.791014237	0.38466385	-1.267480311
49	ENSSSCT00000003175	0.836501268	-0.508146904	0.071790683	-1.407657969
50	ENSSSCT00000003249	0.689109594	1.171045541	1.875958736	0.940837928
51	ENSSSCT00000003332	0.818599679	1.389895073	1.334625163	0.641926361
52	ENSSSCT00000003426	-0.434340638	1.946829341	0.407657969	1.847996907
53	ENSSSCT00000003446	-0.573231301	-0.154251415	0.480166311	-0.059654805
54	ENSSSCT00000003516	0.444930264	0.756589114	-1.696499003	-0.930610405
55	ENSSSCT00000003777	0.971430848	1.889817082	0.634715536	1.938599455
56	ENSSSCT00000003863	1.430634354	1.107528464	1.341036918	-2.021061616
57	ENSSSCT00000004050	-0.151297693	0.445777226	0.075178727	-0.229061285
58	ENSSSCT00000004051	-0.906890596	0.853158612	0.654225163	-1.129767875
59	ENSSSCT00000004168	-0.465380885	-0.196588666	-0.9633052	-0.977670654
60	ENSSSCT00000004237	-2.058893689	-1.125530882	1.584962501	-2
61	ENSSSCT00000004254	0.787006277	1.841302254	1.781359714	1.971985624
62	ENSSSCT00000004309	1.098302074	-0.626382428	0.584962501	-1.491853096
63	ENSSSCT00000004556	-0.914086097	-0.78578151	-2.212993723	-0.957564734
64	ENSSSCT00000004557	0.767248161	1.371385234	0.954833684	0.345496566
65	ENSSSCT00000004568	0.998033135	1.05187603	1.14035964	0.869547178
66	ENSSSCT00000004616	-0.181004926	0.841218643	-1.516132649	-0.701668542
67	ENSSSCT00000004646	0.756728849	-0.454565863	-2.06529146	-1.602664502
68	ENSSSCT00000004748	-0.50688106	-0.021989004	0.554860469	-1.360175564
69	ENSSSCT00000004826	0.503040777	1.08279241	1.095324959	0.738767837
70	ENSSSCT00000004856	-0.56623234	-0.104108403	0.38332864	-1.347923303
71	ENSSSCT00000004957	2.295455884	-0.667424661	-2.807354922	1.064130337
72	ENSSSCT00000005049	-0.150357633	1.11909284	-1.04564996	-0.969679867
73	ENSSSCT00000005197	-1.169925001	-0.326228232	1.557995453	2.938599455
74	ENSSSCT00000005218	-0.421491366	0.853619872	0.061977639	-0.050626073
75	ENSSSCT00000005288	0.392763135	-0.803417516	-1.411581941	-0.826992786
76	ENSSSCT00000005458	-1.284303023	-0.394019718	-3.187627003	-1.791413378
77	ENSSSCT00000005463	0.68775807	0.978168133	1.219829907	0.449202114
78	ENSSSCT00000005475	-0.415037499	0.026176623	-1.321928095	-1.365952719
79	ENSSSCT00000005477	-0.409984272	-0.018642406	-1.241888597	-1.477478074
80	ENSSSCT00000005518	-0.225825613	1.137676312	-0.04699748	0.158211581
81	ENSSSCT00000005545	-0.227640499	-0.674075014	-1.248820547	-1.080565481
82	ENSSSCT00000005744	0.634162207	1.204904995	1.28077077	0.455679484
83	ENSSSCT00000005799	-0.519028231	-0.380167039	-1.917010635	-1.226990656
84	ENSSSCT00000005806	-0.096010885	-0.801900492	-1.535209466	-1.078711976
85	ENSSSCT00000005925	-3.321928095	-1.925999419	-4.523561956	-4.754887502
86	ENSSSCT00000005998	0.703839897	-0.755895323	-1.26052755	0.303069068
87	ENSSSCT00000006041	-1.070389328	0.671243843	0.780218792	1.020464103
88	ENSSSCT00000006064	-0.584962501	-1.730239731	0.814444347	-1.160822794
89	ENSSSCT00000006146	0.492257002	0.592656893	-1.280107919	0.104697379
90	ENSSSCT00000006152	0.341366006	1.253137321	-1.136692272	-0.116193018
91	ENSSSCT00000006160	-0.438217869	-0.426960667	0.644416351	-1
92	ENSSSCT00000006211	0.826463745	0.719190892	-1.495957495	-1.857980995
93	ENSSSCT00000006469	0.719532226	1.120197188	0.929782003	0.974988112
94	ENSSSCT00000006559	0.78181983	-0.354283468	-1.447458977	-1.602036014
95	ENSSSCT00000006611	1.115477217	-0.844721775	0.584962501	0.815575429
96	ENSSSCT00000006651	-0.406838721	-0.213603182	0.116469511	-0.053977621
97	ENSSSCT00000006689	0.446548472	0.661101734	1.24191498	0.57741193
98	ENSSSCT00000006747	2.120093845	-0.398772445	-1.771235496	0.340340132
99	ENSSSCT00000006776	-0.345667682	0.16329037	-1.330010441	-1.034765418
100	ENSSSCT00000006781	3.938599455	2.258068153	0.534336428	0.921997488
101	ENSSSCT00000006801	1.831123088	-0.408805546	-3.906890596	-2.080919995
102	ENSSSCT00000006860	-0.468440123	-0.068576634	0.495768771	-0.987825286
103	ENSSSCT00000006931	0.976321004	-0.790207615	-1.817135943	-1.378511623
104	ENSSSCT00000006989	0.816692787	1.253975067	0.993242634	0.772194082
105	ENSSSCT00000007172	-0.545161493	-0.757429697	-2.157541277	-1.35614381
106	ENSSSCT00000007185	-0.418110595	1.160687912	-2.325510884	-0.745503823
107	ENSSSCT00000007257	0.624354045	0.817500306	-1.029230711	0.529619467
108	ENSSSCT00000007310	-0.126936333	1.224597535	-1.998595917	-0.936806174
109	ENSSSCT00000007380	0.844910927	0.749379765	-0.131644006	0.592017258
110	ENSSSCT00000007413	-0.63634478	-0.006074514	0.191729372	-0.980191519
111	ENSSSCT00000007456	-2.514573173	-3.459431619	2.169925001	3.459431619
112	ENSSSCT00000007532	0.783921353	-0.530514717	-1.33219643	-1.473931188
113	ENSSSCT00000007571	-0.33476091	-0.11589912	-0.146741731	-0.89771895
114	ENSSSCT00000007604	1.698491446	-0.558628353	-1.30256277	-1.781359714
115	ENSSSCT00000007668	-3.584962501	-2.584962501	0.823122238	-3.247927513
116	ENSSSCT00000007969	-0.545520595	-0.471823525	1.111031312	1.206450877
117	ENSSSCT00000008013	-0.584962501	-0.124986777	0.410283969	0.777607579
118	ENSSSCT00000008062	-1.214124805	1.328948523	1.222392421	3
119	ENSSSCT00000008065	-0.714597781	1.914270126	-1.156119202	0.202188851
120	ENSSSCT00000008158	-0.556393349	-1.175849835	0.5360529	-1.607682577
121	ENSSSCT00000008197	-0.945552216	2.121463512	-2.058893689	0.761840263
122	ENSSSCT00000008210	-0.706017668	-0.014613806	-2.123382416	-1.238159737
123	ENSSSCT00000008214	0.898051792	1.207757075	1.704290901	0.802439432
124	ENSSSCT00000008547	1.047025335	1.064425627	-0.725928866	-0.127680647
125	ENSSSCT00000008590	0.497499659	1.410504556	1.93144527	0.858208138
126	ENSSSCT00000008664	-0.37118321	-0.263034406	0.584962501	0.167456746
127	ENSSSCT00000008714	-0.202913413	-0.391469028	0.079659449	0.593786351
128	ENSSSCT00000008741	-0.403396093	0.626541604	-2.953113612	0.890375509
129	ENSSSCT00000008772	1.194172548	1.125530882	1.131244533	1.540568381
130	ENSSSCT00000008845	-3.357552005	0.003499288	-1.182478052	-0.108305009
131	ENSSSCT00000008913	-0.959078532	-0.526620425	0.384917511	-0.831070991
132	ENSSSCT00000008915	-0.224080771	-0.368444805	-0.039528364	0.822001698
133	ENSSSCT00000008959	-0.597792544	-0.356232731	-0.100401897	0.161887682
134	ENSSSCT00000009030	0.53784717	1.213817299	-1.307828987	-0.143069969
135	ENSSSCT00000009047	0.88582898	-1.075767508	-2.079226691	-1.540568381
136	ENSSSCT00000009207	-0.36193509	-0.388597632	0.526249725	0.247180455
137	ENSSSCT00000009251	0.836062358	-0.171800824	-1.284303023	0.344445531
138	ENSSSCT00000009313	-0.721094627	-0.40114202	-0.949016071	-0.816006792
139	ENSSSCT00000009320	0.614193212	1.102482958	-1.381090167	-1.668378509
140	ENSSSCT00000009333	0.494669518	-0.254843071	-1.304061674	-1.103747925
141	ENSSSCT00000009335	-0.317266718	0.845409112	-1.514573173	0.610053482
142	ENSSSCT00000009362	0.945552216	0.800093573	-1.288431183	0.623436649
143	ENSSSCT00000009403	-0.579156879	-0.297680549	-1.63005039	0.868755467
144	ENSSSCT00000009430	-0.202072437	-0.329587472	-1	        -1.134301092
145	ENSSSCT00000009556	-0.293890046	-2.243454037	-1.265142069	0.695145418
146	ENSSSCT00000009565	0.99003889	0.932070146	2.10433666	0.840521786
147	ENSSSCT00000009570	-0.647640665	-0.156429159	0.190521906	0.449802917
148	ENSSSCT00000009590	-1.30580843	-0.247580753	0.668924812	0.485426827
149	ENSSSCT00000009597	0.671377253	1.174497731	1.010239185	0.717469419
150	ENSSSCT00000009679	1.610385098	1.181291256	0.370715168	0.600069393
151	ENSSSCT00000009848	-0.409390936	0.620772577	1.835285855	0.130060541
152	ENSSSCT00000009932	1.402098444	1.164386818	1.736965594	-2
153	ENSSSCT00000010001	-0.265973502	0.58221189	-1.69999396	-0.883485991
154	ENSSSCT00000010007	0.966251209	-0.200024608	-1.55359833	1.334419039
155	ENSSSCT00000010051	-0.416600719	-0.619070355	0.15565087	0.392317423
156	ENSSSCT00000010088	1.326063869	-0.321928095	0.076350886	-1.391190757
157	ENSSSCT00000010101	1.638008735	0.478178159	-1.416646752	-0.972967752
158	ENSSSCT00000010191	0.343919786	-0.281719938	-1.053211806	-0.772302463
159	ENSSSCT00000010220	0.433380079	0.835910282	0.320259275	0.881558543
160	ENSSSCT00000010244	0.989770868	1.303441627	1.426605911	0.718149344
161	ENSSSCT00000010403	3.700439718	1.452512205	-2.485426827	-2.584962501
162	ENSSSCT00000010535	0.686084425	1.272459419	1.459431619	0.869105933
163	ENSSSCT00000010974	-0.619727919	0.899695094	-1.393914208	0.656515762
164	ENSSSCT00000010991	-0.79255792	1.139403057	1.415037499	0.965234582
165	ENSSSCT00000011135	-0.452512205	1.015710083	0.800230488	0.388565288
166	ENSSSCT00000011229	1.280107919	2.115477217	2.222392421	-2.906890596
167	ENSSSCT00000011274	-0.470051601	-0.31169775	0.506235289	0.240478281
168	ENSSSCT00000011441	0.523561956	1.142775253	1.278353456	0.707705565
169	ENSSSCT00000011471	0.63076619	0.618746478	0.311792343	0.088200604
170	ENSSSCT00000011485	0.530093492	1.082048247	1.396566896	0.682900437
171	ENSSSCT00000011504	-1.502500341	2.058893689	1.206450877	1.906890596
172	ENSSSCT00000011568	-0.773931921	-0.46712601	3.227068909	2.402098444
173	ENSSSCT00000011569	-0.069708972	0.662097673	0.479396745	0.432440546
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374	ENSSSCT00000027749	0.815846383	1.843067019	1.744538814	1.046542586
375	ENSSSCT00000027935	-0.374165218	0.884923476	0.661583782	0.813586876
376	ENSSSCT00000027954	-0.53682598	-0.180572246	0.5360529	1.847996907
377	ENSSSCT00000028028	-0.300394933	1.419257966	-2.960829403	-2.271302022
378	ENSSSCT00000028150	-0.449130698	-0.374848409	1.087462841	3.459431619
379	ENSSSCT00000028173	1.523561956	-0.209263149	-1.47883415	-1.257797757
380	ENSSSCT00000028217	-0.874469118	1.541893779	4.129283017	1.447458977
381	ENSSSCT00000028245	3.584962501	-1.129283017	-2.938599455	-3.115477217
382	ENSSSCT00000028273	0.466465738	0.98384752	1.018378529	0.716827949
383	ENSSSCT00000028300	-1.444481277	0.708522888	1.236848276	0.39970784
384	ENSSSCT00000028320	-0.367975462	1.011495639	1.23878686	0.706268797
385	ENSSSCT00000028323	3.169925001	1.651560249	-0.018033923	0.584962501
386	ENSSSCT00000028327	-0.439405506	-0.004815007	-0.034620322	0.121726448
387	ENSSSCT00000028330	2.700439718	1.610318075	1.584962501	2.662965013
388	ENSSSCT00000028340	1.209923069	1.874469118	-1.725825037	-3.392317423
389	ENSSSCT00000028364	1.050626073	0.841302254	0.744742945	0.601450624
390	ENSSSCT00000028373	0.90576393	0.670716648	-1.555646934	0.368722306
391	ENSSSCT00000028510	0.392563637	0.742093663	-1.735829166	0.477897523
392	ENSSSCT00000028524	-1.318489011	-1.859039077	0.102685664	-1.292781749
393	ENSSSCT00000028562	2.263034406	1.03234072	-0.141028025	-1.154246788
394	ENSSSCT00000028657	1.359081093	1.359081093	1.125530882	1.321928095
395	ENSSSCT00000028799	-0.807354922	-0.115426146	-0.282775461	0.11398683
396	ENSSSCT00000028883	2.765534746	1.838015475	0.158262084	0.352881023
397	ENSSSCT00000028905	0.616548844	0.970474048	0.345496566	0.614709844
398	ENSSSCT00000028938	-1.094327383	1.14974712	-3	        1.906890596
399	ENSSSCT00000029012	0.914270126	-1.372156128	0.451106754	1
400	ENSSSCT00000029021	-0.736965594	-1.176877762	0.321928095	-1.745427173
401	ENSSSCT00000029042	-0.836088026	-0.339357901	0.341036918	-1.053938807
402	ENSSSCT00000029112	-0.283984022	-0.943865839	-1.612760953	0.073248982
403	ENSSSCT00000029154	0.403197004	0.956278623	-0.046650093	-1.038328119
404	ENSSSCT00000029211	1.118786152	-0.633758005	-1.267283879	-0.964666927
405	ENSSSCT00000029344	1.830074999	-0.3016557	-2.618909833	1.700439718
406	ENSSSCT00000029353	-1.939259577	-0.196841734	0.460841189	0.189477799
407	ENSSSCT00000029414	-0.277780991	0.68424438	0.578938713	0.328622747
408	ENSSSCT00000029435	0.631791633	1.30765118	1.133430987	0.544604763
409	ENSSSCT00000029512	0.685402402	1.241554678	1.053111336	0.263034406
410	ENSSSCT00000029589	-1.277533976	-1.530514717	1.678071905	-3.700439718
411	ENSSSCT00000029625	0.741007568	-1.554708285	2.271708739	2.578783926
412	ENSSSCT00000029669	1.145050333	1.172315549	-2.093109404	1.253756592
413	ENSSSCT00000029802	0.749766208	1.262036346	1.584962501	0.499707126
414	ENSSSCT00000029842	1.345322317	-0.343372347	-1.084450322	-1.117493563
415	ENSSSCT00000029868	0.827684451	1.389652181	0.37036845	1.064130337
416	ENSSSCT00000029874	0.476054841	0.587946359	-2	       -1.848706022
417	ENSSSCT00000029875	1.043068722	0.731881841	-2.115477217	0.553935605
418	ENSSSCT00000029881	0.665969064	-0.445237737	0.252542032	-1.119298928
419	ENSSSCT00000029889	3.137503524	1.697212727	0.04580369	0.47533801
420	ENSSSCT00000029947	-1.003469409	-0.306661338	0.219713321	0.807354922
421	ENSSSCT00000030122	0.520715577	1.076530937	1.313935304	1.191365526
422	ENSSSCT00000030158	2.087462841	3.700439718	2.874469118	2.222392421
423	ENSSSCT00000030237	-1.38332864	-1.033423002	0.943416472	-3.523561956
424	ENSSSCT00000030332	0.889639553	1.259476586	1.090454951	1.103835811
425	ENSSSCT00000030349	-0.232845697	0.552208171	-1.338689886	-0.163294254
426	ENSSSCT00000030403	0.447817366	1.229481846	0.996095546	0.348921018
427	ENSSSCT00000030455	0.577873076	0.968749066	1.544320516	0.681177816
428	ENSSSCT00000030461	0.438337587	0.022011031	-1.511899039	0.10524316
429	ENSSSCT00000030529	-0.18478296	1.389638323	-1.168453614	-1.155278225
430	ENSSSCT00000030539	-0.185893462	-0.668736918	-0.063286245	0.426442263
431	ENSSSCT00000030715	-0.975752454	-0.672425342	1.932885804	0.815575429
432	ENSSSCT00000030780	0.302180179	0.558618421	-1.279247187	-0.752405349
433	ENSSSCT00000030834	-0.155824804	0.844176175	-1.259452496	-0.141762405
434	ENSSSCT00000030998	-0.212869647	-0.105294943	-1.176877762	-0.99078147
435	ENSSSCT00000031024	-0.85940167	-0.906133077	0.879145605	-1.201633861
436	ENSSSCT00000031054	-1.051614108	-1.107215172	-5.03562391	-2
437	ENSSSCT00000031057	1.053408194	0.516377064	-1.120954209	-0.622897935
438	ENSSSCT00000031089	1.280107919	0.991962681	0.13606155	0.098915026
439	ENSSSCT00000031189	0.6983443	1.34191957	-2.050626073	-2
440	ENSSSCT00000031229	1.453717967	1.060541542	-1.697072867	0.503766418
441	ENSSSCT00000031257	0.573682576	1.16243645	0.722146879	0.247927513
442	ENSSSCT00000031327	-0.742188371	-0.161032991	0.073709686	0.19155961
443	ENSSSCT00000031349	-0.390812263	-0.27966508	-0.181350608	-0.795889736
444	ENSSSCT00000031378	-0.767826558	-0.261741089	0.410283969	-1.292180751
445	ENSSSCT00000031486	1.101879614	0.816485934	2.053866872	0.653824359
446	ENSSSCT00000031617	-0.132643274	1.375672563	-1.172427509	0.589579129
447	ENSSSCT00000031637	-0.653824359	-0.318529518	1.464668267	2.807354922
448	ENSSSCT00000031757	1.632268215	-0.408464845	1.906890596	2.28757659
449	ENSSSCT00000031848	1.262318606	0.702850237	0.811927652	1.422233001
450	ENSSSCT00000031894	-0.225881407	1.090681946	1.296870637	0.85587743
451	ENSSSCT00000031909	0.709353363	1.118090458	0.584962501	0.38172118
452	ENSSSCT00000031970	2.239827015	-1.014888626	-4.575092257	-5.927870535
453	ENSSSCT00000032102	0.535143379	-0.477912774	-1.252063288	0.26589406
454	ENSSSCT00000032138	-0.975752454	0.724365557	0.927850214	1.020464103
455	ENSSSCT00000032249	2.039528364	-1.159656666	-4.694261143	-1.778973121
456	ENSSSCT00000032469	0.815663805	1.307158636	1.869743614	0.602188915
457	ENSSSCT00000032560	1.282108777	1.096948359	-0.989772107	0.220125814
458	ENSSSCT00000032588	-0.689045469	-0.206668069	-0.258221643	0.064868928